摘要
目的:解淀粉芽孢杆菌Q-426在其生长过程中能够产生芬枯草菌素、依枯草菌素、枯草杆菌素等多种脂肽类抗菌物质。利用实时荧光定量PCR的方法考察细菌群体感应信号分子二酮哌嗪类化合物(diketopiperazines,DKPs)对脂肽类抗菌物质合成的调控作用。方法:当Q-426菌进入对数生长期中期,向发酵液中加入终浓度为5 mg/L的DKPs,并继续培养至48 h,并利用实时荧光定量PCR的方法进行抗菌物质mRNA表达水平的定量分析。结果:二酮哌嗪类化合物能够抑制抗菌活性物质相关基因的表达。
Objective： Bacillus amyloliquefaciens Q-426 could produce varieties of antifungal lipopeptides during its stationary growth process,including bacillomycin D,fengycin A and B.Effects of diketopiperazines（DKPs） as signal molecules of quorum sensing（QS） on the biosynthesis of above antifungal compounds were studied through real time fluorescent quantitative polymerase chain reaction（Real-time Q-PCR）.Methods： DKPs at a final concentration of 5 mg/l were added to the culture broth of strain Q-426 which was incubated at 30℃ for 12 h.After continuing cultivation for 48 h,quantitative analysis of mRNA expression levels was carried out by Real-time Q-PCR.Result： DKPs could inhibit the expression of some genes related with the biosynthesis of antifungal lipopeptides.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2012年第3期47-52,共6页
China Biotechnology
