孵育时间在二氧化钛选择性富集磷酸化肽中的影响

Effect of the Incubation Time in the Enrichment of the Phosphopeptides with Titanium Dioxide

目的:旨在探索孵育时间在二氧化钛选择性富集磷酸化肽中的影响。方法:以大肠杆菌BL21(DE3)为材料,预实验确定二氧化钛磁珠与肽段之间的最适比例,之后在此比例下继续探究不同孵育时间条件下的富集效果,以观察孵育时间在二氧化钛富集体系中的影响。结果:预实验确定磁珠与肽段之间最适比例为3∶1,在此比例下发现随着孵育时间的增加其富集效率会逐渐降低,最后确定大肠杆菌中最适孵育时间为5min。结论:孵育时间在大肠杆菌二氧化钛富集体系中对富集效果有明显的影响,并且呈负相关。推测不同样品的最佳孵育时间与样品种属有关。

Objective： Aims to explore the effect of the incubation time in the enrichment of the phosphopeptides with Titanium dioxide.Methods： Firstly,the optimum ratio between beads and peptides based on the preliminary study is ascertained,and then compare the effect of the incubation time on the enrichment with Titanium dioxide.Results： The optimal ratio between bead and peptide is 3∶1.Under this optimized ratio,the longer incubation time decreases bead selectivity and 5 minutes is the optimal incubation time.Conclusion： The incubation time has the negative effect on the phopshopeptide enrichment which indicates that the optimal time depends on sample species.