摘要
miR-17-92是一个高度保守的基因家簇,参与哺乳动物多个器官发育并与多种实体瘤的发生密切相关。运用多个在线数据库,发现了miR-17-92的上游转录因子及下游靶基因间的多个前馈和反馈环路。并对参与miR-17-92调控环路的基因进行功能聚类分析,进而绘制出miR-17-92的核心调控网络图。结果提示miR-17-92与其上游转录因子共调控的靶基因可能参与了生物体的细胞周期调控,迁移、凋亡、激素应答、免疫系统发育等多种生物学过程,KEGG pathway分析提示其还与多种肿瘤信号通路密切相关。因此,对miR-17-92分子调控网络生物信息学的分析可以有助于理解其在细胞发育和肿瘤发生过程中的作用机制并为后续实验验证提供良好的指导。
miR-17-92 cluster is highly conserved and involved in the development of diverse organs and solid tumors in mammal.Several transcriptional factors and target genes are found,which regulated and be regulated by miR-17-92,formed some feed-forward and feed-back loops via different online databases.After functional cluster analysis of genes involved in the circus regulated by miR-17-92,the regulation network responses to miR-17-92 has been drafted out.The anlysis indicates that the jointargets of miR-17-92 and its upstream transcriptional factors may play important roles in the biological processes such as cell cycle,metastasis,apoptosis,hormone responses and the development of immune system.KEGG pathway analysis also implied that it was related to many signalling pathways in tumors.The study will be a good guideline for the further research and provide great facility for understanding the mechanism of cell development and tumorousgenesis.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2012年第3期69-75,共7页
China Biotechnology
基金
国家自然科学基金资助项目(81071524)
