可发酵糖为底物不同菌种丁醇生产能力的研究

Study on the Ability of Butanol Production of Different Bacteria with the Fermentable Sugar

随着新一代生物质能源的研发,利用梭菌的发酵生产丁醇已成为热点。选用能生产丁醇的Clostridium acetobutylicum AS1.7,Clostridium acetobutylicum AS1.132,Clostridium acetobutylicumAS1.134和Clostridium beijerinckii NCMIB 8052,在多种糖源下进行发酵培养,通过比较其在不同糖源条件下的生长情况、糖利用率、丁醇及副产物产量、对丁醇、木糖耐受能力等,综合筛选出了最适用于发酵生产丁醇的备选菌种。NCMIB8052因具有最高产量、相对优良的耐受性及可利用多种糖源的特点,而被确定为发酵能力最强的菌种。

With the development of the new generation of biomass energy,it has become a popular topic that to use fermentable sugar and Clostridia to produce butanol.The study is going to use Clostridium acetobutylicum AS1.7,Clostridium acetobutylicum AS1.132,Clostridium acetobutylicum AS1.134 and Clostridium beijerinckii NCMIB 8052,which can produce butanol in various sources of sugar by fermentation.By comparing growth behaviors,ratio of using sugars,yield of butanol and by-product,and endurance of butanol and xylose,we are going to find out the most suitable Clostridium,which can be used to the industrial.In the experiment,NCMIB8052 is the most outstanding Clostridium as it has the highest yield,relative high endurance and the ability using multiple sugars.