基因组重排技术在开发新代谢产物中的应用

The Application of Genome Shuffling in Developing New Metabolites

基因组重排是一种基于原生质体融合,并对原生质进行递推式融合的新型技术。随着基因组重排技术的不断发展和成熟,通过基因组重排获得新代谢产物的例子不断出现,表明该项技术作为新代谢产物开发的途径具有一定的应用前景。在此列举了基因组重排在开发新代谢产物方面的成果,包括基因组重排激活沉默基因产生新代谢产物;基因组重排引入单酶基因产生新抗生素;基因组重排互换基因模块产生杂合抗生素和基因组重排替换前体基因产生新抗生素的例子,并展望了其发展的趋势。

Genome shuffling is a new-type technology based on protoplast fusion and recursive protoplast fusion.With the development and mature of genome shuffling,many new metabolites have been obtained through this technology,which demonstrates a promising prospect for genome shuffling to be a way to develop new metabolites.The new metabolites developed by genome shuffling technology are focused,including metabolites from activation of silent genes,new antibiotics from introduction of single enzyme gene,hybrid antibiotics from exchange of gene modules and new materials from replacement of precursor gene.The prospect of this technology is also discussed.