摘要
目的:观察人参皂甙Rd缺血前预给药对大鼠局灶性脑缺血/再灌注损伤后多聚ADP核糖聚合物含量的影响。方法:60只健康雄性Sprague-Dawley大鼠,体重280～300 g,随机分为假手术组(Sham组)、丙二醇组(Vehicle组)和人参皂甙Rd处理组(Rd组),每组20只。Vehicle组和Rd组大鼠采用MCAO线栓法阻塞大鼠右侧大脑中动脉,2 h后拔出栓线达到再灌注目的,建立急性局灶性脑缺血/再灌注模型。Vehicle组和Rd组分别于造模前30 min腹腔注射丙二醇(人参皂甙Rd稀释液)和人参皂甙Rd(10 mg/kg)。Sham组手术操作同前,但线栓未阻塞大脑中动脉。Western Blot和免疫组织化学方法检测大鼠大脑中动脉阻塞4 h后多聚ADP核糖聚合物的含量。结果:与Sham组相比,Vehicle组和Rd组缺血侧脑组织PAR聚合物含量增加(P<0.01);与Vehicle组相比,Rd组缺血侧脑组织PAR聚合物含量明显上调(P<0.01)。结论:10 mg/kg人参皂甙Rd预处理可能通过增加PAR聚合物的含量起到脑缺血损伤早期神经保护作用。
Objective： To investigate the effect of Ginsenoside Rd pretreatment on poly（ADP-ribose）（PAR） polymer expression in rats after focal cerebral ischemia.Methods： A total of 60 male Sprague-Dawley rats weighing 280-300 g were randomly divided into sham operation group（Sham group）,propanediol group（Vehicle group） and ginsenoside Rd pretreatment group（Rd group）,with 20 in each group.The focal cerebral ischemia/reperfusion was induced by intervening a MCAO monofilament from the right external carotid artery into the origin of the middle cerebral artery and removing it after 2 h.In Vehicle group and Rd group,rats were respectively exposed to propanediol and ginsenoside Rd（10 mg/kg） for 30 min before the occlusion of the right middle cerebral artery.The rats in Sham group were operated the same as propanediol group,but cerebral artery was not blocked.The expression of poly（ADP-ribose） polymer was detected by Western-Blot and immunohistochemistry at 4 h after MCAO in rats.Results： Our results showed that PAR level in Vehicle group and Rd group were increased compared with the one in Sham group（P〈0.01）;PAR level in Rd group was significantly improved compared with the one in Vehicle group（P〈0.01）.Conclusion： The results suggest that neuroprotection of Ginsenoside Rd（10 mg/kg） may be related to the increase of the expression of poly（ADP-ribose） polymer at the early stage of focal cerebral ischemia injury.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2012年第2期183-186,共4页
Chinese Journal of Neuroanatomy
