细粒棘球蚴囊液对HepG2肝癌细胞系细胞周期的影响

Effect of Echinococcus granulosus cyst fluid on mitogen-activated protein kinases and the cell cycle of host cells in vitro

目的探讨细粒棘球蚴(Eg)囊液(HCF)在感染急性期对宿主肝细胞MAPK信号通路及细胞周期的影响。方法常规方法培养HepG2细胞,以HCF为刺激物,于不同时间点用Western blot检测p-ERK、增殖细胞核抗原PC-NA、细胞周期蛋白cyclin-D1、A、B1和E的表达水平,并与无FCS组比较。结果 Western blot检测显示,15%、30%HCF组p-ERK分别在换液培养3h和30min高表达(P<0.05),PCNA分别在3h和12h高表达(P<0.05),cyclin-D1在3h高表达(P<0.05),cyclin-A分别在3h和12h高表达(P<0.05),15%HCF组cyclin-E在3h高表达(P<0.05),15%、30%HCF组cyclin-B1在各时间点表达量微弱(P<0.05)。结论 HCF对体外培养的HepG2细胞无毒害,且对其增殖有一定的促进作用。

Objective To discuss the effect of Echinococcus granulosus cyst fluid on mitogen-activated protein kinases and the cell cycle of host cells in the acute phase of infection.Methods HepG2 cells were cultured normally and stimulated with hydatid cyst fluid（HCF）.Western blot indicated the expression of p-ERK,PCNA,cyclin-A,cyclin-B1,cyclin-D1,and cyclin-E at different times and results were compared to those of cells not in the presence of FCS.Results Western blot showed that cells stimulated with 15%HCF and 30%HCF had a high level of p-ERK expression at 3 h and 30 min（P〈0.05）,a high level of PCNA expression at 3 h and 12 h（P〈0.05）,a high level of cyclin-D1 expression at 3 h（P〈0.05）,and a high level of cyclin-A expression at 3 h and 12 h（P〈0.05）.Cells stimulated with 15%HCF had a high level of cyclin-E expression at 3 h（P〈0.05）.Cells stimulated with 15%HCF and 30%HCF had a low level of cyclin-B1 expression at every test time（P〈0.05）.Conclusion HCF did not inhibit primary cultured host cells but did promote the proliferation of host cells.