丹参多酚酸盐对ApoE基因敲除小鼠血清脂质的干预作用

Effects of salvianolate on serum lipid levels in ApoE^(-/-)mice

目的:观察中药丹参多酚酸盐对高脂饮食ApoE-/-小鼠脂质代谢的影响,探讨丹参多酚酸盐抗动脉粥样硬化作用机制。方法:将4周龄雄性C57BL/6J ApoE-/-小鼠喂食高脂饮食,随机分为4组。模型组腹腔注射生理盐水、丹参多酚酸盐干预分为低剂量组(60 mg/kg)、中剂量组(120 mg/kg)和高剂量组(240 mg/kg)腹腔注射丹参多酚酸,另设C57BL/6J野生型小鼠为对照组。于24周末时处死各组小鼠,留取血清,检测各组小鼠血清总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)水平。结果:与对照组比较,ApoE-/-鼠血清TC、LDL-C均明显升高(P<0.05),表明敲除ApoE基因能导致血脂代谢紊乱。应用丹参多酚酸盐后,各干预组TC、LDL-C水平均明显降低(P<0.05),但TG和HDL-C水平无明显改变,中、高剂量组尤为显著。结论:丹参多酚酸盐可降低ApoE-/-小鼠血清TC和LDL-C水平,可能是其抗动脉粥样硬化的作用机制之一。

Objective：To investigate the anti-atherosclerotic effects of salvianolate （Sal）, a Chinese medical herb, on serum lipid levels in Apo E-/- mice fed with high fat diet. Methods： Twenty-four C57BL/6J Apo E -/1 mice （4-week old male） were randomly divided into four groups according to intrapertoneal injection of saline （Saline group）, 60mg/kg （low-dose group）, 120 mg/kg （middle- dose group）, or 240mg/kg （high-dose group） of Sal. Another five C57BL/6J wild mice served as normal controls. At the end of 24 weeks, all animals were sacrificed, and serum levels of total cholesterol （TC）, triglyceride （TG）, high-density lipoprotein （ HDL-C）, and low-density lipoprotein （LDL-C） were assessed by a RX-2000 Automatic biochemical analyzer. Results：Compared with normal controls, serum levels of TC and LDL C were significantly higher in the Apo E-/- mice. After treatment with Sal, serum levels of TC and LDL-C were decreased, which was more obvious in the middle- and high-dose groups. Serum levels of TG and HDL-C were not significantly changed. Conclusion： Sal can decrease serum levels of TC and LDL in Apo E-/1 mice, suggesting its anti-atherosclerotic effects.