摘要
目的制备抗邻苯二甲酸单苄酯(mono-benzyl phthalate,MBzP)的单克隆抗体。方法本实验利用活泼酯法,将邻苯二甲酸单苄酯分别与载体蛋白BSA和OVA偶联,获得完全抗原MBzP-BSA和MBzP-OVA,用MBzP-BSA免疫Balb/c小鼠,制备杂交瘤细胞,利用ELISA方法检测阳性杂交瘤细胞株分泌抗体的效价。结果获得了稳定分泌抗MBzP的细胞株1B9,以该细胞株体内诱生腹水制备腹水型单克隆抗体,纯化后效价为1∶128 000。结论成功制备了抗邻苯二甲酸单苄酯的单克隆抗体。
Objective To prepare monoclonal antibody against MBzP（mono-benzyl phthalate）.Methods MBzP was linked with vector protein BSA and OVA to produce the completed antigen（MBzP-BSA and MBzP-OVA）.Balb/c mice were immunized with MBzP-BSA to make hybridoma cells.Use ELISA to detect the titer of antibody which secreted by the hybridoma cells.Results The cell line 1B9 which can steadily produce monoclonal antibody against MBzP was obtained.The titer of the purified ascite was 1∶128 0000.Conclusion Successfully obtained the monoclonal antibody against MBzP.
出处
《中国实验诊断学》
2012年第3期417-419,共3页
Chinese Journal of Laboratory Diagnosis
基金
国家自然科学基金项目(31071539)
吉林大学农学部引进人才科研启动项目(4305050102A8)
