基质金属蛋白酶-9联合尿脱落细胞FISH检测诊断膀胱癌的价值

Significance of detecting FISH and scrum MMP-9 in the bladder urothelial carcinoma

目的探讨基质金属蛋白酶-9和尿脱落细胞荧光原位杂交联合检测诊断膀胱癌的价值。方法膀胱移行上皮细胞癌患者100例(TCCB组),同期健康体检者20例(对照组),均应用ELISA法检测血清基质金属蛋白酶-9水平,荧光原位杂交技术检测尿脱落细胞表达,比较2组检测结果。结果 TCCB组基质金属蛋白酶-9水平,尿脱落细胞阳性率均高于对照组(P<0.05)。联合检测可明显提高膀胱移行上皮细胞癌检出率。结论基质金属蛋白酶-9和尿脱落细胞荧光原位杂交联合检测对膀胱移行上皮细胞癌诊断有重要参考价值。

Objective To inevestigate the value of detecting scrum metalloptoteinase-9（MMP-9） and fluorescence in situ hybridization（FISH） in diagnosis of the Bladder urothelial carcinoma.Methods 20 cases of healthy adults and 100 cases of patients with bladder cancer were involved.and effectiveness of in diagnosis of the Bladder urothelial carcinoma.The nucleus of shedding cells were detected by FISH and Serum MMP-9 contents were detected in 100 patients with transitional cell carcinoma of bladder and 20 healthy controls by enzyme-linked immunosorbent assay（ELISA）.Results The levels of both MMP-9 and FISH in cases of malignant the Bladder urothelial tumors were significantly higner as compared with those of the benign and controls（P〈0.01） and The positive staining had no relevance to age,gender,and the type of tumor（P〉0.05）.Conclusion Combined MMP-9 and FISH detection is of important value in diagnosisi of the Bladder urothelial tumors and may bridge the gap of single determination FISH in differential diagnosis.