番茄黄化曲叶病毒的快速分子检测

Molecular detection of tomato yellow leaf curl virus (TYLCV)

番茄黄化曲叶病毒是当前世界范围内危害番茄生产的毁灭性病害。文章针对番茄黄化曲叶病毒全基因组序列的特异区段自主设计了1对特异性PCR引物(上游引物TYLCV-F:5′-ACGCATGCCTCTAATCCAGTGTA-3′,下游引物TYLCV-R:5′-CCAATAAGGCGTAAGCGTGTAGAC-3′),依据PCR扩增特异片段543 bp的有无可以快速、准确、高效、特异地检测出是否感染了TYLCV病毒,这项技术可以方便地应用到工厂化育苗的带毒性检测、蔬菜大规模生产中植株发病情况的快速检测以及抗病毒育种,从而为蔬菜安全可持续生产提供科技支撑。

Tomato yellow leaf curl virus（TYLCV） is currently considered as one of the most devastating viruses in cultivated tomatoes（Solanum lycopersicum） worldwide.We reported here the development of a PCR-based method to quickly detect TYLCV using the primer pairs（TYLCV-F： 5＇-ACG CAT GCC TCT AAT CCA GTG TA-3＇ and TYLCV-R： 5＇-CCA ATA AGG CGT AAG CGT GTA GAC-3＇）,which was designed based on the genome sequence of TYLCV.A TYLCV-specific band of 543 bp was amplified from infected tomato plants.This protocol provides a rapid,reliable,and sensitive tool for molecular detection and identification of TYLCV in the industrial seedling and virus resistance breeding to facilitate safe and sustainable production of tomato.