LPT技术在非妇科领域应用价值的研究

Application value of LPT in the research of nongynecology cell examination

目的通过对两种制片方法所做各种非妇科标本的分析研究,探讨薄层液基细胞学制片技术(LPT)在临床非妇科领域的应用价值。方法对1185例临床送检的非妇科标本同时分别进行传统涂片法及LPT液基细胞学检测,研究两种涂片的制片质量和诊断结果,数据采用卡方检验。结果 LPT制片与传统涂片相比,前者细胞数量多、核染色质着色鲜明可靠、成团细胞中的单个细胞界限分明,染色背景清晰;传统涂片细胞核染色质着色均一、缺乏立体感,染色背景欠清晰。LTP技术在涂片质量上明显优于传统涂片,两种方法差异有统计学意义(P<0.01)。LPT制片诊断可疑恶性32例,恶性59例,阳性率7.7%;传统涂片诊断可疑恶性16例,恶性29例,阳性率3.8%。两种制片方法的恶性细胞诊断率差异有统计学意义(P<0.01)。结论 LPT技术克服了传统涂片假阴性率高的缺点,提高了恶性肿瘤的检出率及准确性,具有重要临床意义,值得推广应用。

Objective To study the value of Liquid-based preparation cytology test system（LPT） in the research of nongynecology cell examination by means of the analysis and study of the nongynecology subjects which are made through two kinds of ways of making plates.Methods 1185 cases of nongynecology cell examination were detected simultaneously and respectively using traditional way and LPT.The smear quality and diagnosi results of the two groups were compared and analyzed with chi-square test.Results Comparing with the LPT and the traditional cells smears,the former had the dominances of plentiful cells,vivid colouring of nuclear chromatin,distinctmargin of single cell in mass cells,and clear background.LTP technology was better than traditional smears in smear quality,and the difference was significant compared with the control group（P0.01）.Of these 1185 cases,the doubtful carcinomatous cases diagnosed by LPT technology were 32,the carcinomatous cases were 59,the positive rate was 7.7%.The doubtful carcinomatous cases diagnosed by the traditional cells smears were 16,the carcinomatous cases were 29,the positive rate was 3.8%.There was obvious difference in the diagnosis in malignant cells between them（P 0.01）.Conclusion LPT technology overcomes the traditional cells smears false negative rate of shortcoming,can increase the rate of identifying the malignant cells and more accurately,,can be widely used.