葛根素(普乐林)对SHRsp脑卒中预防和治疗作用的研究

Effects of Puerarin on Stroke in Stroke-prone Spontaneously Hypertensive Rats

目的：研究普乐林注射液对卒中型自发性高血压大鼠（ＳＨＲｓｐ）脑卒中的预防和治疗作用。材料和方法：将８０只８周龄（雌雄各半）ＳＨＲｓｐ随机分为普乐林 ２００ ｍｇ／ｋｇ，１００ｍｇ／ｋｇ及对照组，饮 １． ０％～１． ５％ ＮａＣｌ盐水。实验一：在高盐负荷开始即灌胃治疗，８周后断头处死，取颈动脉及脑组织做电镜检查。取脑组织光镜下检查脑卒中发生率。实验二：脑卒中发生后开始灌胃治疗，连续１０天。记录大鼠脑卒中临床表现分数及存活天数。结果：实验一：治疗后普乐林 ２００ ｍｇ／ｋｇ， １００ ｍｇ／ｋｇ组颈动脉电镜扫描和脑组织电镜透射观察组织病理结果均好于对照组；光镜下脑卒中检出率（４１．７％，５０．０％）也低于对照组（７６．９％）。实验二：治疗前脑卒中临床表现分数三组间相似，治疗后普乐林 ２００ ｍｇ／ｋｇ， １００ ｍｇ／ｋｇ组分数（０． ９５±１． ３９，２．４１±１．６５）均明显低于对照组（３．７７±０．２７）（Ｐ＜０．０１～０． ００１）。治疗期存活天数普乐林 ２００ ｍｓ／ｋｇ组（９． ７３±１．０３）及１００ ｍｇ／ｋｇ组（７．９３±２．６４）均长于对照组（５．３６±４．４５）（Ｐ＜０． ０５， Ｐ＞０． ０５）。脑与心脏重量?

Aim: The study is to investigate the preventive and treatment effects of puerarin (PR) on stroke in strokeprone spontaneously hypertensive rats (SHRsp). Methods:Eighty SHRsp of 8 week old were randomly divided into PR 200 mg/kg, 100 mg/kg and control group. Rats were given 1. 0% - 1. 5 % NaCl as drinking fluid ad libido. In ex- periment 1: rats were given PR 200 mg/kg·dm^(-1), 100 mg/kg· d^(-1) or water control at the beginning of experiment for 8 weeks. The histopathologic examination of their brain and carotid artery was made after decapitation. In experiment 2: after onset of stroke, rats were given orally PR 200 mg/kg· d^(-1), 100 mg/kg ·d^(-1) or water control for 10 days. The clinical score of stroke and survival time of rats were recorded. Results: In experiment 1: the incidence of stroke by microscopy in PR 200 mg/kg and 100 mg/kg group was 41. 7% and 50. 0% respectively, that was decreased than control group (76. 9% ). Electron microscope study showed the endothelium of carotid artery were normal in 200 mg/kg group, while it was seriously injured in control group and mildly injured in PR 100 mg/kg group. The brain morphological study showed normal in PR 200 mg/kg group, significant neuron damage were found in the control. In experiment 2: the clinical scores of stroke after treatment with PR 200 mg/kg and 100 mg/kg group were 0. 95 ± 1. 39 and 2. 41 ± 1. 65 respectively, that is lowered than control group (3. 77 ± 0. 27, P<0. 01). The survival time in PR 200 mg/kg 9. 7± 1. 0 day and PR 100 mg/kg 7. 9± 2. 6 day that is longer than control group (5. 36± 4. 45 day,P<0. 05). Conclusion:PR may prevent and effectively treat the stroke in SHRsp.