苯那普利对SHR大鼠左心室细胞外基质的影响

Effects of Benazepril on the Extracellular Matrix of Left Ventricle in Spontaneously Hypertensive Rats

目的：探讨苯那普利（一种血管紧张素转换酶抑制剂）是否具有将自发性高血压大鼠（ＳＨＲ）左心室肥厚（ＬＶＨ）时增生的胶原和其它细胞外基质成份恢复到正常水平的作用。方法：６周龄的雄性ＳＨＲ给予口服苯那普利（１０ ｍｇ·ｋｇ^（－１）·ｄ^（－１））１６周，性别、年龄配对的ＳＨＲ和Ｗｉｓｔａｒ－Ｋｙｏｔｏ（ＷＫＹ）大鼠作为对照。测量血压、体重、左心室肥厚的指标，采用免疫组化方法作纤维粘连蛋白（ＦＮ）、层粘连蛋白（ＬＮ）和ＩＶ型胶原染色，计算机图像半定量分析。结果：（１）苯那普利治疗组ＳＨＲ的ＳＢＰ和ＬＶＭ／ＢＷ比显著低于对照组ＳＨＲ；（２）治疗组ＩＶ型胶原、ＦＮ和ＬＮ含量也显著低于未治疗组。结论：苯那普利可减轻ＳＨＲ大鼠左心室肥厚的发展，伴有心肌细胞外基质的减少。

Aim: To evaluate the potential benefit of the angiotensin coverting enzyme inhibitor (ACEI ) benazepril in reversal of the proliferation of collagen and other extracellular matrix(ECM) components in the myocardium of spontaneously hypertensive rats (SHR). Methods: 6-week-old male SHRs were given benazepril (10mg ·kg^(-1)·d^(-1) ) for 16 weeks. Sex and age matched SHR and WKY served as controls. The body weight (BW ), systolic blood pressure (SBP), left ventricular mass (LVM ) were examined. Myocardial deposition of collagen type IV and other ECM components (fibronectin, laminin) was analyzed by immunohistochemical techniques using specific antibodies. Results: (1)The SBP and LVM/BW in benazepril treatment SHR were significantly lower than that of control SHR; (2) Collagen type IV, fibronectin and laminin was significantly decreased in benazepril treated SHR compared with control. Conclusion:These results indicate that benazepril could attenuate the progression of left ventricular hypertrophy in SHR, which is associated with a decrease in ECM component in the myocardium.