摘要
目的研究人颞叶内侧癫痫海马组织星形胶质细胞水通道蛋白4(AQP4)和内向整流性钾离子通道4.1(Kir4.1)的分布。方法对10例颞叶内侧癫痫(MTLE)和6例非颞叶内侧癫痫(non-MTLE)手术切除海马组织,应用光镜及透射电镜观察组织学及超微结构,免疫荧光组织化学法观察星形胶质细胞AQP4和Kir4.1的分布。结果 MTLE海马组织星形胶质细胞大量增生伴明显肿胀,神经元显著固缩。non-MTLE海马组织中,AQP4和Kir4.1在星形胶质细胞血管周围足突(pAST-ef)分布多于其他部位,呈现极性分布特点;而在MTLE海马组织中,AQP4和Kir4.1在pAST-ef分布减少,其他部位分布增加,极性分布改变。结论海马组织星形胶质细胞上AQP4和Kir4.1极性分布变化可能与颞叶内侧癫痫发生相关。
Objective To investigate the distribution of aquaporin 4（AQP4） and inwardly rectifying potassium channel 4.1（Kir4.1） in the astrocytes from human mesial temporal lobe epilepsy（MTLE）.Methods Hippocampal specimens,including 10 cases of MTLE and 6 cases of non-MTLE,were observed under optical and transmission electron microscopy.The distribution of AQP4 and Kir4.1 in astrocytes was investigated with immunoflurescence.Results Compared with non-MTLE hippocampus,the main structural changes of MTLE included remarkable hyperplasia astrocytes,serious swelling astrocytes and distinguished astrophy neurons.In non-MTLE hippocampus,immunoflurescence signals of AQP4 and Kir4.1 were enriched along perivascular astrocyte end-feet domain.However,it reveals significant loss of AQP4 and Kir4.1 in perivascular astrocyte end-feet domain in MTLE.Conclusion Loss of perivascular AQP4 and Kir4.1 in the human MTLE may help to understand the roles of astrocyte in MTLE.
出处
《中国康复理论与实践》
CSCD
北大核心
2012年第3期215-218,共4页
Chinese Journal of Rehabilitation Theory and Practice
基金
国家自然科学基金资助项目(30840083)
