抑制人肝星状细胞株迁移的中药组分筛选

Screening Chinese medicine components for inhibition of human hepatic stellate cell migration

目的从6种已证实有抗肝纤维化作用的中药组分中,筛选出抑制肝星状细胞迁移的最佳者。方法培养人肝星状细胞株(LX-2),用细胞迁移杯培养方法分别观察不同浓度梯度(10-4、10-5、10-6、10-7mol/L)的青蒿琥酯、灯盏花素、红景天苷、丹参酚酸B盐、粉防己碱、黄芪总甙对血小板衍生生长因子-BB(PDGF-BB)(10 ng/ml)诱导的LX-2迁移的抑制,从而筛选出各组分最佳作用浓度;随后比较各组分最佳浓度对LX-2迁移的抑制作用,而筛选出最佳组分。结果 6种中药组分均可抑制PDGF诱导的LX-2的迁移;但10-4mol/L丹参酚酸B盐、10-4mol/L黄芪总甙、10-4mol/L与10-5mol/L粉防己碱对LX-2形态有不同程度的影响;依照药物筛选原则,最终选出10-4mol/L青蒿琥酯、10-4mol/L灯盏花素、10-5mol/L红景天甙、10-5mol/L丹参酚酸B盐、10-6mol/L粉防己碱、10-6mol/L黄芪总甙为最佳浓度;将各组分最佳浓度进行统一比较证实,10-5mol/L红景天苷具有更好的抑制迁移作用。结论 10-5mol/L红景天苷抑制PDGF诱导的LX-2迁移的作用最佳。

Objective To screen the best component from six of components of traditional Chinese medicine which were confirmed with the anti-fibrosis function.Methods A trans-well chamber system was used to observe the changes of the human hepatic stellated cell line（LX-2） migration.We select human HSC cell line（LX-2） which induced by platelet derived growth factor-BB（PDGF-BB） to study the effect of inhibition of migration.Cells were incubated with 10-4mol/L-10-7mol/L concentration of Artesunate,Breviscapine,Salidroside,Salvianolic acid B,Tetrandrine,Astragalosides,respectively.Results Six kinds of Chinese medicine components can inhibit the PDGF-induced migration of LX-2.10-4mol/L Salvianolic acid B,10-4mol/L Astragalosides,10-4mol/L Tetrandrine and10-5mol/L Tetrandrine have different affectiveness on cell morphology.Based on the principles of the selection,10-4mol/L Artesunate,10-4mol/L Breviscapine,10-5mol/L Salidroside,10-5mol/L Salvianolic acid B,10-6mol/L Tetrandrine and 10-6mol/L Astragalosides can significantly inhibit LX-2 migration.Of six of them,10-5mol/L Salvianolic has the best effectiveness（P〈0.05）.Conclusion 10-5mol/L Salidroside is the most effective drugs that inhibit the migration of LX-2.