肉苁蓉中麦角甾苷含量的测定方法

A Method for Simlultaneous Determination of Acteoside in Herbe Cistanches

用HPLC法测定肉苁蓉中麦角甾苷的含量.采用Kromasil C18（4.6 mm×250 mm,5μm）色谱柱,以甲醇-水（V（甲醇）∶V（水）=35∶65）为流动相（水中含有0.4 mL/L磷酸）等质洗脱,流速1 mL/min,检测波长331 nm,柱温35℃.检测结果表明：麦角甾苷在0.34~2.04μg范围内呈良好线性关系,回归方程为A=192.57C-2.896（r=0.999 9）,平均回收率为98.1%,RSD为1.99%（n=5）.本方法用于测定肉苁蓉中麦角甾苷的含量,重现性良好、结果准确可靠.

A HPLC method for simlultaneous determination of acteoside in herbe cistanches was established.Agilgent 1100 system and Kromasil C18（4.6 mm×250 mm,5 μm）were adopted.The mobile phase consisted of H2OCH3OH（65∶35）and the detection wavelenth was 331 nm with a PDA detector.The flow rate was 1 mL/min and the column temperature was 35 ℃.The linear ranges of acteoside was 0.34～2.04 μg,the average recovery was 98.1 %,RSD was 1.99 %（n=5）.This method is accurate and reliable.It can be used to determine the content of acteoside in herbe cistanches.