红豆杉浸膏在氧化铝催化下紫杉醇的生成研究

Studies of Taxol Convertible from Taxus Extract Catalyzed with Al_2O_3

目的 :研究在氧化铝层析初分离红豆杉浸膏中紫杉醇增量的来源。方法 :采用C18 硅胶反相层析、氧化铝层析及硅胶层析等方法对物料进行处理 ,HPLC分析紫杉醇的含量。结果 :紫杉醇增量主要来源于红豆杉浸膏中 7 表 紫杉醇的碱性氧化铝催化下的异构化。结论 :优化 7 表 紫杉醇的异构化条件 ,可以增加 7 表 紫杉醇向紫杉醇的转化。

Purpose:To study the resource of the increased of taxol during the Al_2O_3 chromatography to initial separation of taxol from Taxus Yunnanensis extract.Methods:The extract was treated with C 18 silica gel reversed phase chromatography,Al 2O 3 chromatography and silica gel chromatography.The content of taxol was determined by HPLC.Results:The main resource of the increased taxol was the isomerization of 7 epi taxol under the catalysis of Al_2O_3.Conclusion:7-epi-taxol could be more convertible to taxol under the optimized conditions of the isomeric reaction.