人非小细胞肺癌组织p73基因转录表达研究

Study on the transcript expression of p73 gene in human non small cell lung cancer tissues

目的 探讨p73基因转录表达与肺癌发生、发展的关系。方法 采用逆转录多聚酶链反应技术 (RT PCR)检测 3 2例非小细胞肺癌组织、癌旁组织和 7例肺良性病变组织 ,及其对应正常肺组织p73mRNA的表达。结果 3 2例肺癌组织中 2 8例p73表达明显升高 ,肺癌组织中p73mRNA阳性表达率明显高于癌旁肺组织和肺良性病变组织 (P <0 .0 1)。肺癌组织中p73mRNA的阳性表达率与组织学类型、细胞分化程度和P TNM分期无明显关系 (P >0 .0 5 )。结论 肺癌组织中存在p73基因的过度转录表达 ,它可能参与调控非小细胞肺癌的发生。

Objective To explore the relationship among the p73 gene transcript expression and the oncogenesis, development of human non small cell lung cancer (NSCLC). Methods The expression of p73 mRNA was detected in 32 human NSCLC tissues, para cancer tissues, 7 lung benign lesion tissues and matched non cancer lung tissues by semiquantitative reverse transcriptase polymerase chain reaction (RT PCR).Results Overexpression of p73 mRNA was found in 28/32 NSCLC tissues. The positive expression rate of the p73 mRNA in the cancer tissues was significantly higher than that in the para cancer tissues and benign lesion tissues either (P<0.01). No significant relationship was observed among p73 expression and differentiated grades, histoclassification and P TNM stages of the cancer (P>0.05). Conclusion The results suggest that there is an overexpression of p73 mRNA in lung cancer tissues and it may paly an important role in the tumorigenesis and development in NSCLC.