p16基因及产物在原发性肺癌中的表达及其临床病理学意义

The clinicopathologic significance of the p16 gene expression in primary lung cancer

目的 探讨p16基因及产物的异常表达与肺癌发生、发展的关系。方法 应用免疫组化检测 114例原发性肺癌组织中p16蛋白的表达 ,应用PCR技术分析 62例p16蛋白表达丢失的肺癌组织中p16外显子 2 (E2 )的缺失情况 ,并以 14例癌旁正常肺组织作对照。结果 p16蛋白阳性表达率在鳞癌、腺癌和小细胞肺癌中分别为 43 .2 %、48.5 %和 2 7.0 % ,各组间无显著差异 (P >0 .0 5 ) ;p16蛋白表达水平与非小细胞肺癌 (NSCLC)的组织学分级和淋巴结转移有密切关系。NSCLC中p16E2的缺失率为45 .2 % ,缺失水平与淋巴结转移和组织学分级密切相关 (P <0 .0 5 )。小细胞肺癌 (SCLC)和癌旁正常肺组织中无p16E2的缺失。结论 p16基因及产物的异常表达与NSCLC的组织学分级和转移相关 ,它可能参与NSCLC的发生。

Objective To explore the relationship between p16 gene expression and oncogenesis, and development of human lung cancer. Methods Immunohistochemistry was employed to detect the expression of p16 protein in 114 paraffin embedded primary lung cancer tissues. Loss of p16 INK 4 gene exon 2 (E2) was analyzed using PCR technique in 62 cancer samples with p16 protein negative stain and 14 paracancerous lung tissues. Results The positive rates of p16 protein expression in squamous cell carcinoma, adenocarcinoma and small cell lung cancer (SCLC) were 43.3% , 48.2% and 27.0% respectively, and no significant difference was found among them (P> 0.05 ). The expression level of p16 protein was closely correlated with histological grade and lymph node metastasis of lung cancer. The deletion of p16 E2 was 45.2% in NSCLC and closely related to lymph node metastasis and histological grade (P<0.05). No deletion was observed in 37 cases of SCLC and 14 paracancerous lung tissues. Conclusion Alteration of p16 gene and protein is closely related to the histological grade and lymph node metastasis of NSCLC. It may play an important role in the oncogenesis, development and lymph node metastasis of NSCLC.