具抗肿瘤活性海洋细菌HY108的发酵优化

Fermentation optimization of a marine bacteria strain HY108 with anti-tumor activity

目的对海洋细菌HY108进行发酵工艺的优化,以提高菌株发酵液的抗肿瘤活性。方法采用MTT检测法检测菌株发酵液对人肝癌细胞SMMC-7721的抑制率,对该菌株的培养基组成和发酵条件进行了确定。结果通过对该菌株产抗肿瘤活性物质的发酵培养基和发酵条件的优化,菌株发酵液对肿瘤细胞的抑制率提高了近20%,为提高抗肿瘤先导化合物的产率提供了理论依据。结论通过优化,获得最佳发酵培养基为:玉米粉3%、葡萄糖0.5%、蛋白胨0.2%和酵母粉1.2%,pH6.5～7.0;最佳发酵条件为:种龄24h,以2%的接种量接种,28℃的条件下150r/min振荡培养3d。

Objective To advance the antitumor activity of fermentation broth by the fermentation optimization of strain HY108.Methods By MTT method,the inhibit rate of fermentation against human liver cell SMMC-7721 was researched.And the compounds of culture medium and fermentation condition was studied.Results By study of fermentation technology,its inhibit rate against human liver cancer cell SMMC-7721 raised 20% to provide the theory basis for enhancing productionrate of antitumor forerunner compounds.Conclusion By study of fermentation technology,the optimized medium consisted of 3% maizena,0.5% glucose,0.2% peptone,1.2% yeast powder,pH6.5～7.0.The optimum culture condition was：seed cultural time 24h,2% inoculation amount,28℃,150r/min for 3d.