高氧暴露对新生大鼠肺SP-C表达的影响

Effect of hyperoxia on the expression of surfactant protein C in newborn rats

目的探讨高氧暴露对新生大鼠肺组织表面活性蛋白C(SP-C)表达的影响。方法将新生的72只SD大鼠随机分为空气组、高氧组,每组36只。每组又分为3、7、14d三个亚组。于空气或高氧暴露后3、7、14d取肺组织,采用免疫组化方法观察肺组织中SP-C表达的变化。结果高氧组3d SP-C表达强度较空气组增强(P<0.05),高氧组7dSP-C表达强度与空气组比较,差异无统计学意义(P>0.05),高氧组14d SP-C表达强度较空气组减弱,差异有统计学意义(P<0.01)。结论高氧暴露可导致新生大鼠肺SP-C表达减弱或功能缺失,SP-C表达异常可能是高氧肺损伤的重要因素。

Objectives To explore the effect of hyperoxia on expression of surfactant protein C（SP-C）in newborn rats.Methods The 72 newborn SD rats were randomly divided into air group and hyperoxia group.Each group has 36 rats.The rats in every group were randomly into the 3rd,7th,14th day subgroups respectively.The sub-groups of 3rd,7th and 14th were cut lung tissue in the corresponding of time,expression of SP-C protein was detected by immunohistochemistry.Results The expression of SP-C in hyperoxia group was stronger than air group in 3rd day,P0.05;The expression of SP-C showed no significant difference between hyperoxia group and air group in 7th day,（P0.05）,Significant difference was noticed between hyperoxia group and air group in 14th day,（P0.01）.Conclusion Hyperoxia exposure lead to a down regulation or functional impairment of the SP-C expression,this may be an important factor for hyperoxia lung injury.