世界卫生组织和世界动物卫生组织禽流感检测流程的比较

Comparsion of avian influenza detection systems established by WHO and OIE

目的了解世界卫生组织(World Health Organization,WHO)和世界动物卫生组织(Office International DesEpizooties,OIE)的禽流感检测流程的差异,旨在相互借鉴,以建立更加完善的检测流程。方法重点比较WHO和OIE推荐的禽流感RT-PCR检测流程中对于初步阳性、可疑和阴性的样本处理程序,以及发出报告的确证程序,来探讨和完善科学的检测模式。结果 WHO的禽流感检测流程中为防止假阴性结果,还需要进行人流感病毒的进一步检测,为防止假阳性,需要进行测序确证;OIE的禽流感检测流程中为防止假阴性结果,还需要再次采样进行确证,为防止假阳性,还需要与病毒分离培养结果综合定论。结论 WHO和OIE的检测流程都能有效的防止假阴性和假阳性的结果产生,但仍需要补充和完善禽流感其他亚型检测流程。

Objective To understand the differences of the detective systems of avian influenza,one of them established by world health organization（WHO）,and the other by office international des epizooties（OIE）.And to improve the processes of this kind of system based on learning the advantages from each other.Methods Through comparing the procedures of both of systems detecting initial positive,suspicious and negative results and the procedure confirming issued reports,exploring and perfecting the detecting model on the basis of scientific concept.Results Detecting process of WHO needed to undertake further detection of human influenza virus to prevent the false negative results and utilized sequencing confirmed to prevent the false positive.Detecting process of OIE required sampling again to prevent the false negative results and needed aggregate analysis on consequences of virus isolation experiment to prevent false positive results.Conclusion Both of the detecting processes are effective to prevent false negative and false positive results,but they still need to be improved by supplementing other avian influenza subtype detecting process.