雌激素对实验性骨质疏松症骨折愈合的影响

Effects of estrogen on fracture healing of experimental osteoporotic mice

目的 研究不同剂量的雌激素对卵巢摘除后发生骨质疏松小鼠股骨骨折愈合的影响。方法成年 balb/ c小鼠卵巢摘除后 3个月开始制作右股骨中段闭合骨折模型 ,同一天开始皮下注射高低两种剂量雌激素 ,小鼠于折骨后 5、10、15、2 0、30天分批处死 ,采用 X射线、骨痂称重、骨痂中钙盐沉积率测定、组织学检查、血清生化检测等方法研究雌激素对骨折愈合的影响 ,并和卵巢摘除组、假手术对照组进行比较。结果 卵巢摘除组骨痂较小 ,但软骨痂钙化 (骨痂成熟 )时间未受到明显影响。高剂量雌激素可使软骨痂钙化时间推迟 ,干扰了骨痂成熟过程 ;生理剂量的雌激素对骨痂成熟影响较小。结论 雌激素缺乏和补充超生理剂量雌激素均对去势小鼠骨折早期愈合不利 ,补充生理剂量雌激素对小鼠骨折愈合影响较小。

Objective To investigate the effects of estrogen on fracture healing of ovariectomized mice with serious bone loss. Methods Adult balb/ c mice underwent fracture of right femurs at middle point three months after ovariectomy and began to receive low and high doses of estrogen.Animals were sacrificed at 5 ,10 ,15 ,2 0 and 30 days after fracture.X- rays,callus weighing,calcium depositing rate mea- surement,histological study and serun biochemical examination were used to investigate the effects of e- strogen on fracture healing,and to compare with ovariectomized ( OVX) group and sham- operated con- trol( SO) group. Results The calluses were small in OVX group,but the callus maturity was not affect- ed.High dose of estrogen impaired cartilage callus calcification while low dose of estrogen affected callus formation little. Conclusion Estrogen deficiency and super- physiological level of estrogen both interfere with fracture healing of ovarietomized mice.Physiological level of estrogen interferes with fracture heal- ing little in ovariectomized mice.