锌对铅染毒新生大鼠成骨细胞增殖分化的影响

Combined Effects of Lead and Zinc on Proliferation and Differentiation of osteoblasts in Newborn Rats

目的:探讨铅锌联合染毒对乳鼠颅骨成骨细胞增殖分化的影响。方法:分离并培养原代成骨细胞,加入不同浓度铅、锌培养48h,检测其对成骨细胞增殖的作用;用碱性磷酸酶试剂盒检测ALP活力。结果:在染铅48h后,当铅浓度≥10μmol/L时,细胞增殖功能下降(P<0.05);加锌干预48h后,铅+锌组细胞增殖功能均高于各自单独染铅组,其中铅(1μmol/L、10μmol/L)+锌(50μmol/L)组、铅(10)+锌(100)组与对照组间的差异具有统计学意义(P<0.05)。铅干预48h后,100μmol/L铅组的ALP活力显著下(P<0.05),给予锌干预的铅锌联合染毒组,各组ALP活力均有增加,其中铅(1μmol/L、10μmol/L)+锌(50μmol/L)组ALP活力均高于对照组,而铅(100μmol/L)+锌(50μmol/L)组ALP活力低于对照组,差异均有统计学意义(P<0.05)。结论:铅对成骨细胞有毒性作用,影响其增殖和分化功能;50μmol/L锌在一定程度上可以拮抗铅对成骨细胞增殖和分化功能的损伤,且对ALP活力的作用更显著,为铅中毒骨病的防治提供一定的科学依据。

Objective： To investigate the effects of lead and zinc on proliferation and differentiation of osteoblasts in newborn rats.Methods： OsteobLasts were isolated from the calvaria of newborn rats and cultured in α-MEM medium.Lead and Zinc acetate with different concentration was administrated and cultivated for 48h.The proliferation response to lead and zinc was determined by MTT methods.The activity of alkaline phosphatase（ALP） was measured by alkaline phosphatase kit.Results： Treated with different levels of lead（10 μmol/L and 100 μmol/L） for 48h,the cell proliferation decreased（P〈0.05）;Treated with zinc and lead for 48h,cell proliferation was higher than their single lead-treated group;differences were of statistical significance（P〈0.05） between the lead（1 μmol/L） plus zinc（50μmol/L）,lead（10μmol/L） plus zinc（50μmol/L）,lead（10μmol/L） plus zinc（100μmol/L） and control group.The activity of ALP was significantly decreased in group treated with a dose of 100 μmol/L lead for 48h（P〈0.05）.When treated with zinc and lead for 48h,the activity of ALP was higher than their single lead-treated group;the ALP activity in groups of lead（1μmol/L,10μmol/L） plus zinc（50μmol/L） were higher than the control group,while that in group of lead（100 μmol/L） plus zinc（50 μmol/L） was significantly lower than the control group.All the difference was statistically significant（P〈0.05）.Conclusions： There were adverse effects of lead on osteoblasts such as affecting their proliferation and differentiation.Zinc（50μmol/L） may have antagonistic joint action on lead in affec-ting the proliferation and differentiation of osteoblasts.Moreover,it also has a notable role on the activity of ALP.So it provides some proofs in preventing and curing lead poising bone diseases