摘要
目的:研究癫痫模型大鼠中prepro-orexin及其神经纤维在不同时间点的变化情况,以阐明prepro-orexin在癫痫发生中的作用,深化癫痫的发病机制。方法:本研究采用海人酸腹腔注射诱发大鼠癫痫发作,并分别于癫痫终止后8小时、1、3、7天和慢性复发时间点行免疫组化方法检测prepro-orexin免疫反应阳性细胞数及其神经纤维的变化情况。结果:prepro-orexin免疫阳性细胞的分布主要在外侧下丘脑和穹窿周围核,在海马、大脑皮层及其他大脑组织中并未检测到,各组之间prepro-orexin阳性细胞数进行方差分析表明其差异并不显著,P>0.05;而其免疫阳性神经纤维主要分布在下丘脑、丘脑室旁核及海马,数量稀少;结论:大鼠致痫后,随着时间的推移,prepro-orexin免疫阳性细胞总体减少,但未具有统计学意义,但并不能完全认为其与癫痫发作无关;而其免疫反应神经纤维稀少。
Objective： To investigate the changes of the prepro-orexin and its nerve fibers in the rat epilepsy model at different time points.Methods： Seizures were induced by intraperitoneal injection of kainic acid.The changes of the prepro-orexin immunoreactive cells and nerve fibers were detected by immunohistochemistry at 8 hours,1,3,7 days and chronic relapsing time after the termination of epilepsy respectively.Results： Prepro-orexin immunoreactive cells distributed mainly in the hypothalamus and perifornical nuclei,and were not detected in the hippocampus,cerebral cortex and other brain tissues.Its immunoreactive nerve fibers distributed sparsely in the hypothalamus paraventricular thalamic nucleus and the hippocampus;Conclusion： The overall reduction of the prepro-orexin immunoreactive cells is not statistically significant,but can’t be fully considerde that they have nothing to do with the seizure.And their immunoreactive fibers distribut sparsely.
出处
《现代生物医学进展》
CAS
2012年第4期619-622,共4页
Progress in Modern Biomedicine
基金
国家自然科学基金(30971025)
