核因子-κ B在腹部火器伤肠管穿透后肺组织细胞凋亡中的作用

The Role of Nuclear Factor Kappa B in Pneumocyte Apoptosis Induced by Intestinal Perforations Due to Abdominal Firearm Wound

目的:探讨腹部火器伤肠管穿透后核因子-κB在肺组织细胞凋亡中的作用,了解腹部火器伤肠管穿透后继发性肺损伤机制。方法:健康长白仔猪42头随机等分为对照组和伤后1h、2h、4h、8h、12h和24h组,实验组建立腹部火器伤肠管穿透模型后,用免疫组化图像分析法测定各组肺组织内NF-κB活性,同时用TUNEL测定肺组织细胞凋亡变化情况。结果:伤后各组肺组织内NF-κB活性明显高于对照组,并于伤后8h出现高峰。肺组织细胞凋亡指数伤后显著增高(P<0.05),并与NF-κB活性变化基本一致。结论:腹部火器伤肠管穿透后肺组织内NF-κB活性增强,肺细胞凋亡增多,从而继发导致肺损伤。

Objective： To investigate the effect of NF-κ B in signal transduction of pneumocyte apoptosis in order to investigate the mechanism of lung injury.Methods： 42 Chang-Bai piglets were randomized into 7 groups： control group wounded 1hour group,2hour group,4hour group,8hour group,12hour group and 24hour group.The model of intestinal perforations due to abdominal firearm wound was established in wounded groups.Pulmonary NF-κ B activity was detected by immunohistochemical staining and image analysis in all groups.Hepatocyte apoptosis indexes were detected.Results： Levels of pulmonary NF-κ B activity in wounded groups were significantly elevated compared with that in control group,and it appeared one peaks in 8h group.Meanwhile,pneumocyte apoptosis indexes increased markedly compared with that in control group（P＆lt;0.05）.All these changes were positive correlation with the changes of pulmonary NF-κ B activity.Conclusion： After intestinal perforations duing to abdominal firearm wound,pulmonary NF-κ B activity increases and it can induce pneumocyte apoptosis and lung injury.