百草枯中毒大鼠TNF-α、IL-10的表达及大黄保护作用的研究

The Study on Expression of TNF-α、IL-10 in Rats with Paraquat Intoxication and the Protection Effect of Rhubarb

目的:观察大黄对急性百草枯中毒大鼠TNF-α、IL-10的干预作用,探讨其可能的作用机制。方法:90只SD大鼠随机分为生理盐水对照组(A组)、PQ(60 mg/kg)灌胃染毒组(B组)、生大黄(300mg/kg.d)干预组(C组),每组30只。中毒后6h、24h、72h分批处死存活的大鼠,并且检测大鼠血浆TNF-α、IL-10水平。结果:B组、C组TNF-α、IL-10水平在染毒后6h开始升高,72h达到高峰,与A组相比,差异有统计学意义(P<0.05、P<0.01),在相同时间点C组TNF-α和IL-10的表达低于B组,差异均有统计学意义(P<0.01)。B组、C组血浆TNF-α、IL-10水平与中毒时间呈显著正性相关关系(r=0.849,P<0.01;r=0.790,P<0.01;r=0.0.943,P<0.01;r=0.892,P<0.01)。结论:大黄能够通过降低百草枯中毒大鼠体内的TNF-α、IL-10水平,减轻百草枯对大鼠的损伤作用。

Objective： To evaluate the effect of rhubarb on tumor necrosis factor-a（TNF-α） and interleukin-10（IL-10） in rats with paraquat intoxication,and investigate its mechanism.Methods： 90 healthy adult SD rats were randomly divided into Group A（control group n=30）,Group B（poisoned group n=30）,Group C（rhubarb-treated group n=30）.Group B and C were treated intragastrically with PQ at 60 mg/kg,Group C given rhubarb at 300mg/kg.d,while only saline was treated intragastrically the other two groups.Live rats in each group were sacrificed at 6 hours,24 hours and 72 hours,for the determination of TNF-α,IL-10 in plasma of rats.Results： The leves of TNF-α,IL-10 increased at 6 hours after paraquat intoxication in Group B and Group C,and peaked at 72 hours later,with statistical difference from Group A（P＆lt;0.05,P＆lt;0.01）;Compared with group B,delayed,lower increasing extent,obviously reducing tendency in group C,with statistical difference（P＆lt;0.01）.As to the leves of TNF-α,IL-10and poisoning time,there were high positive correlations among them.Conclusions： Rhubarb ameliorates damage effect caused by PQ poisoning in rats by means of inhibiting the expression of TNF-α,IL-10 in turn to alleviate inflammatory reaction.