用斜入射光反射差法无标记实时监测不同浓度兔IgG和山羊抗兔IgG反应的动力学过程

Label-free and real-time detection of interactional dynamic processes of rabbit IgG with different concentrations and goat anti-rabbit IgG by oblique-incidence reflectivity difference method

用斜入射光反射差法无标记实时监测1.25 mg/mL,2.50 mg/mL和5.00 mg/mL三种不同浓度兔IgG和浓度为0.02 mg/mL山羊抗兔IgG反应的动态过程,同时监测和获得三种不同浓度IgG反应的动力学曲线以及相应的反应时间.实验结果表明,斜入射光反射差法不仅能无标记地判别生物分子之间是否发生反应,而且能无标记实时监测生物分子反应的动力学过程,在生物分子相互作用的研究方面具有潜在和广泛的应用前景.

We successfully label-free and real-time detecte the interactional dynamic processes of rabbit IgG with different concentrations （1.25 mg/mL,2.50 mg/mL and 5.00 mg/mL）,and goat anti-rabbit IgG with a concentration of 0.02 mg/mL by the oblique-incidence reflectivity difference method（OIRD）.The dynamic curves and times of interaction,response to concentration of rabbit IgG,are obtained.The experimental results demonstrate that the OIRD method can not only can distinguish the biomolecular interactions,but also real-time detect the interactional dynamic processes of biomolecules,indicating that the OIRD method has promising and extensive applications for the investigating of biomolecular interactions.