生物技术及美拉德反应改良烟梗提取液的性质

Improvement of the nature of tobacco stem extracts by biotechnology and Maillard reaction

利用酶、微生物以及美拉德反应对烟梗提取液处理以改善烟梗提取液性质,并与传统的三级逆流提取工艺进行对比。烟梗提取液经一系列酶处理后,后续经两种工艺处理,工艺一:美拉德反应后微生物发酵;工艺二:微生物发酵后美拉德反应。对比三级逆流提取工艺,工艺一所得最终提取液,还原糖减少了3.0%,氨基态氮增加了41%,挥发性物质增加了165.3%;工艺二所得提取液还原糖增加了3.9%,氨基态氮增加了54%,挥发性物质增加了239.6%。确定工艺二为较合理的改善烟梗提取液性质的工艺路线。

In order to raise the use value of tobacco stems,tobacco stem extract was treated by applying enzymes, microbes and Maillard reaction for improving the nature of tobacco stems, which was compared with traditional three-stage countercurrent extraction technology.After being treated by a series of enzymes,tobacco stem extract was handled with other two processes： the first process, maillard reaction following microbial fermentation~ the second process, microbial fermentation following Maillard reaction. Comparing with traditional three- stage countercurrent extraction technology,in the final extraction obtained by the first process,the content of reducing sugar decreased 3.0% ,the content of amino nitrogen increased 41% and the content of volatile matters increased 165.3%.While the content of reducing sugar,amino nitrogen and volatile matters in the final extraction through the second process increased 3.9% ,54% and 239.6%, respectively.So the second process was determined as the more reasonable route to improve the tobacco stem extract.