基于通径分析酶解啤酒糟提取蛋白质

Zymolysis brewer’s spent grain for extracting protein by path analysis

为了提取啤酒糟中的蛋白质,采用碱性蛋白酶酶解啤酒糟。在单因素实验中用纤维素酶预处理啤酒糟,结果降低了蛋白质的提取率。采用通径分析第一轮均匀实验,表明增加酶量、调高pH、延长酶解时间,有利于提高蛋白质的提取率,同时误差项对蛋白质的提取率存在较大的影响。在通径分析的基础上,进行第二轮均匀实验,通过二次多项式逐步回归分析得出最佳的酶解条件,酶量3400u/g、料液比1∶13、时间5.0h、pH8.5,其蛋白质的提取率为64.15%,比第一轮提高了28.04%。

In order to extract protein from brewer＇ s spent grain,the alkali protease was used in hydrolyzing brewer＇ s spent grain.Brewer＇s spent grain was pretreated by cellulose enzyme,which reduced protein extraction rate in single-factor experiment.It was shown that increasing enzyme volume, heightening pH and prolonging enzymolysis time,were helpful to improve protein extraction rate,meanwhile,error term great influenced on protein extraction rate in the first uniform design experiment by path analysis. Based on path analysis results, the second uniform design experiment was adopted, through quadratic polynomial stepwise regression analysis, optimal enzymatic hydrolysis condition was obtained ： enzyme volume 3400u/g, ratio of material and water 1 ~ 13, time 5.0h,.pH 8.5, protein extraction rate was 64.15% ,improved by 28.04% than the first uniform design experiment.