樟子松疱锈病重寄生菌的分离鉴定

Isolation and identification of the fungi parasitizing Cronartium flaccidum in Pinus sylvestris var.mongolica

樟子松疱锈病是樟子松枝干上的主要病害,为利用重寄生菌对樟子松疱锈病进行生物防治,对其病原菌松芍柱锈菌Cronartium flaccidum(Alb.et Schw.)Wint.的重寄生菌进行了分离和鉴定,得到重寄生菌拟枝孢镰刀菌Fusarium sporotrichioides Sherb.、深绿木霉Trichoderma atroviride,并测定ITS序列,获得基因登录号。经显微观察发现接种重寄生菌后锈孢子受到不同程度的破坏,且存在两种不同的作用机理。

Blister rust was one of the most severe diseases in Pinus sylvestris var. morgolica forest. Isolation and identification of mycoparasites on Cronartium flaccidum were conducted to control the disease with mycoparasites. The results showed that Fusarium sporotrichioides and Trichoderma atroviride were mycoparasites on Cronartium flaccidum, and the NCBI numbers were got to identify their taxonomic status, Microscopic observation of aeciospores showed that different mycoparasites had different effects on aeciospores.