8种药食兼用原料的总酚与总黄酮及DPPH自由基清除活性

The Total Phenolic and Flavonoid Contents and DPPH Scavenging Capacity of Eight Chinese Edible Herbal Extracts

采用Folin-Ciocalteu法、紫外分光光度法、DPPH自由基活性(DRA)分析法测定酸枣仁、栀子、甘草、罗汉果、决明子、莱菔子、菊花、郁李仁8种药食兼用植物原料的总酚含量、总黄酮含量以及DPPH自由基清除能力。结果表明:总酚含量较高的为甘草、决明子、菊花(>20mg/g),较低的为栀子、罗汉果、酸枣仁、郁李仁、莱菔子;总黄酮含量较高的为决明子、菊花、栀子(>24mg/g),较低的为甘草、酸枣仁、郁李仁、罗汉果、莱菔子;DPPH自由基清除能力较高的为栀子、菊花(>85%),较低的为莱菔子、罗汉果、甘草、郁李仁、决明子、酸枣仁。

Folin- Ciocaheu method, ultraviolet spectrophotometry and DPPH radical -scavenging capacity analytical method were employed to test the total phenolic and flavonoid contents and the DPPH scavenging capacity of 8 Chinese edible herbal materials. The results showed that in the eight Chinese edible herbal materials, Glycyrrhiza uralensis Fisch, Cassia obtusifolia L and Chrysanthemum morifolium Ramat showed the highest total phenolic contents （ 〉 20mg/g）. Gardenia jasminoides Ellis, Momordica grosvenori Swingle, Semen ziziphi Spinosae, Prunus japonica Thunb and Raphanus sativus L＇s total phenolic contents were low. Cassia obtusifolia L, Chrysanthemum morifoliu. Ramat and Gardenia jasminoides Ellis showed the highest total flavonoid contents（ 〉 24mg/g） while Glycyrrhiza uralensis Fisch, Semen ziziphi Spinosae, Prunus japonica Thunb, Momordica grosvenori Swingle and Raphanus sativus L are low. Gardenia jasminoides Ellis and Chrysanthemum morifolium Ramat have the hinghest capacity in clearing DPPH free radical. Raphanus sativus L, Momordica grosvenori Swingle, Glycyrrhiza uralensis Fisch, Prunus japonica Thunb, Cassia obtusifolia L and Semen ziziphi Spinosae＇s capacity of clearing DPPH free radical is lower.