新型多酸化合物对人乳腺癌细胞增殖的抑制作用

The growth inhibition of human breast cancer cells induced with new polyoxometalates

采用MTT法、形态学方法和Western blot方法,检测了3种新型多酸化合物[SbW9、(SbW9)2-(SnR)4、(SbW9)2-(SnR-CH3)4]对人乳腺癌MCF7和MDA-MB-231细胞生长抑制作用,细胞形态学变化及细胞PCNA蛋白表达变化,并探讨该3种化合物抑癌的机制.实验结果表明:3种化合物对人乳腺癌细胞MCF7和MDA-MB-231的生长均具有明显的抑制作用(P<0.01),细胞形态发生明显变化,细胞皱缩并且增殖速度明显减慢;其中,(SbW9)2-(SnR)4可使MCF7细胞PCNA蛋白表达下降(P<0.05),且呈浓度剂量依赖性.说明该3种新型多酸化合物均具有抗肿瘤活性,其活性部位可能是以{SbW9}为基本建筑单元的聚阴离子,作用机制可能与其抑制细胞DNA的合成有关.

The purpose of the current study is to investigate the growth inhibition and the mechanism of MCF7 and MDA-MB-231 cells induced with new trivacant Keggin-type polyoxometalates [SbW9、（SbW9）2-（SnR）4、（SbW9）2-（SnR-CH3）4],and explore the underlying molecular mechanism.The antiproliferative effective of compounds on MCF7 and MDA-MB-231 cells was measured with MTT.Morphological changes were observed by microscopic.The protein expression of PCNA was determined by Western blotting.MTT assay shows that proliferation of MCF7 and MDA-MB-231 cells were significantly inhibited,the compounds（P〈0.01）.Microscopic observation of cell morphology changed significantly,and the cells were shrinked and the proliferation were reduced significantly.In particular,the expression of PCNA protein was decreased significantly（P〈0.05） in a dose-dependent manner by（SbW9）2-（SnR）4.The results of the study indicated that the decisive site of anti-tumor activity which has SbW9 as its basic building unit maybe relate to inhibition of cell complexes on DNA synthesis.