TACE治疗对兔VX2肾移植瘤细胞增殖影响的实验研究

Experimental studies on cell proliferation after transcatheter arterial chemoembolization of VX2 carcinoma implanted in rabbit kidney

目的：探讨经肾动脉化疗栓塞术（TACE）对兔VX2肾移植瘤模型增殖细胞核抗原（PCNA）表达的影响,评价兔VX2肾移植瘤介入治疗的疗效。材料与方法：将瘤块种植到22只新西兰纯种大白兔的左肾上极。超声监测肿瘤生长,待肿瘤长至1~2 cm大小时,将实验兔随机分为对照组、TACE组,对照组10只,实验组12只。行TACE术：对照组（A组）给予生理盐水10 mL;TACE组（B组）给予超液化碘油0.2 mL/kg和阿霉素2 mg/kg。栓塞后7 d处死动物取出瘤块,用10%中性福尔马林液固定,石蜡包埋,5μm连续切片,免疫组化染色检测肿瘤PCNA的表达。结果：本实验免疫组化结果显示2组共22只实验动物中PCNA均有表达,阳性率为100%,TACE组（71.6±11.01）%的细胞增殖指数（PI）明显高于对照组（61.2±11.21）%（P=0.041）。结论：经导管动脉化疗栓塞术是一种创伤小、疗效确切的兔VX2肾移植瘤治疗方法;TACE术后1周残存肿瘤组织细胞PI升高。

Objective： To investigate the therapeutic effect and the expression of proliferating cell nuclear antigen（PCNA） in renal implantation tumor after transcatheter arterial chemoembolization（TACE）.Methods： VX2 tumor was inoculated directly into the exposed kidney by tumor blocks embedding in twenty-two New Zealand white rabbits.When the diameter of tumors reached 1~2 cm,the rabbits bearing tumor were randomly divided into two groups,control group（group A n=10） and experimental group（group B n=12）.For group A,normal saline was administered via hepatic artery.For group B,lipiodol（0.2 mL/kg） and ADM（2 mg/kg） were administered for each rabbit.Seven days after embolism,the rabbits were sacrificed and the tumors were taken out.All specimens were fixed by 10% neutral formalin,embedded with paraffin completely and consecutive sections were made with a 5 μm-thickness.Then immunohistochemistry was performed to demonstrate the expression of PCNA.Results： PCNA was expressed in 22 animals in two groups（the postive rate=100%）.The PI（71.6±11.01）% of residual tumor cells of group B was higher than that of group A（61.2±11.21）%（P0.05）.Conclusions： TACE is a minor trauma and effective treatment for rabbits VX2 renal tumor,the proliferation of residual tumor cells is increased effectually in experimental group.