肺癌组织中前列腺素E合成酶和环氧化酶的表达及生物学意义

Expression and biological significance of mPGES-1 and COX-2 in lung cancer

目的探讨前列腺素E合成酶（mPGES-1）和环氧化酶（COX-2）在肺癌组织中的表达及生物学意义。方法采用RT—PCR检测肺癌及癌旁组织中mPGES-1、COX-2mRNA的表达，同时采用免疫组织化学S—P法检测相应标本中mPGES-1和COX-2蛋白的表达。结果肺癌组织中mPGES-1和COX-2mRNA的表达量均明显高于癌旁组织（均P〈0．05）；mPGES-1蛋白阳性表达率在肺癌组织中为66．7％（40／60）明显高于相应癌旁组织中的10．0％（6／60）（x2=20．38，P〈0．01）；COX-2蛋白的阳性表达率在肺癌组织中为73．3％（44／60）明显高于在相应的癌旁组织中的13．3％（8／60）（X2=21．99，P〈0．01）。肺癌组织中mPGES-1和COX-2蛋白的阳性表达率与肿瘤大小、组织类型无关（均P〉0．05），而与淋巴结转移、TNM分期、分化程度等有关（均P〈0．05）。结论mPGES-1和COX-2的高表达可能在肺癌的发生发展中起着重要作用，可能为肺癌的早期诊断和开发肺癌的靶向治疗提供一定的临床依据。

Objective To explore the expression and biological significance of microsomal prostaglan din E synthase-1 （ mPGES-1 ） and cyclooxyenase-2 （COX-2） in lung cancer. Methods The expressions of mPGES-1 and COX-2 mRNA in cancerous tissue and tumor-adjacent tissue were detected by RT-PCR,while the immunohistochemi- cal S-P method was used to evaluate the expression of mPGES-1 and COX-2 proteins in the corresponding tissues. Results The mRNA level of COX-2 and mPGES-1 significantly increased（P 〈 0.05） in lung cancer group as com- pared with those in the tumor-adjacent tissue. The positive expression rate of mPGES-1 in cancerous tissue and tumor- adjacent tissue were 66.7% （40/60） and 10.0% （6/60） respectively. The positive expression rate of COX-2 protein in cancerous tissue and tumor-adjacent tissue were 73.3% （44/60）and 13.3% （8/60） ,respectively. The positive expression rate of COX-2 was significantly higher in lung cancer than in tumor-adjacent tissue （ X2 = 21.99, P 〈 0.01 ）. The positive expression rate of mPGES-1 and COX-2 protein in lung cancer was independent of size of tumor and histological type （ P 〉 0.05 ）, but correlated with histological grade, lymph node metastasis and TNM staging. （ P 〈 0. 05 ）. Conclusion It suggested that the expression of mPGES-1 and COX-2 in lung cancer may play an important role in the process of carcinogenesis, and may provide a clinical basis for the early diagnosis and targeted therapy of lung cancer.