SSAO抑制剂对LPS诱导的巨噬细胞p65核移位的影响

THE EFFECTS OF SSAO INHIBITORS ON P65 TRANSLOCATION INDUCED BY LPS

目的研究肼屈嗪(HYD)、氨基脲(SEM)、LJP1207、氨基胍(AMI)及二溴乙胺(BEA)五种不同的氨基脲敏感型胺氧化酶(SSAO)抑制剂对脂多糖(LPS)诱导的小鼠腹腔巨噬细胞NF-κB p65蛋白核移位的影响。方法分离培养Balb/c小鼠腹腔巨噬细胞,分别加入含0、10、50、100、200、500μm HYD、SEM、LJP1207、AMI或BEA的培养基,同时加入LPS使之终浓度为10μg/m1,作用2 h。免疫荧光法观察几种SSAO抑制剂对LPS诱导的NF-κB p65移位的影响。结果静息状态巨噬细胞p65主要定位于胞浆中,LPS可引起小鼠巨噬细胞p65核移位。HYD、SEM、AMI、BEA、LJP1207均对LPS诱导的p65核移位有不同程度的抑制作用。结论 SSAO抑制剂可抑制LPS诱导的巨噬细胞p65核移位,该作用可能与其抗炎作用有关。

Objective To investigate the effects of semicarbazide -sensitive amine oxidase （SSAO） inhibi- tors （HYD, SEM, LJP1207, AMI or BEA） on murine peritoneal macrophages NF- KB 1）65 translocation. Methods Isolated murine peritoneal macrophages were cultured with 10μg/mL lipopolysaccharide （LPS） for 2h, with/without different concentrations of HYD, SEM, LJP1207, AMI or BEA. After incubation, p65 translocation was observed with immumofluorescence method. Results p65 was located in cytoplasm in unstimulated cells, while translocated into nucleus after stimulated by LPS. HYD, SEM, LJP1207, AMI or BEA could inhibit p65 translocation. Conclusion SSAO inhibitors could inhibit p65 translocation induced by LPS, which might explain their anti - inflammatory response in another aspect.