摘要
目的探讨辛伐他汀对人肾小管上皮细胞(HKC)上内皮细胞蛋白C受体(EPCR)的影响。方法采用流式细胞仪检测HKC上EPCR的表达,并将肿瘤坏死因子-α(TNF-α)和辛伐他汀作用于HKC,采用RT-PCR技术对上述因子作用后的HKC上EPCR mRNA表达进行检测。结果 EPCR在HKC中有高表达,TNF-α可下调HKC中EPCR mRNA的表达,而辛伐他汀可以明显改善TNF-α对EPCR的抑制。结论 HKC中EPCR的表达可受到TNF-α的影响,辛伐他汀对上述影响因素有一定拮抗作用,对肾小管上皮细胞损伤可能有保护作用。
Objective To investigate the effect of Simvastatin on endothelial protein C receptor（EPCR） in Human Renal Tubular Epithelial Cells（HKC）.Methods The expresson of EPCR in HKC was detected by flow cytometry treated by tumor necrosis factor alpha（TNF-α） and Simvastatin.The expression of EPCR in HKC was detected by RT-PCR.Results The results showed that the expression of EPCR were significantly increased in HKC,and the level of EPCR in HKC was suppressed by TNF-α.Simvastatin can obviously reverse the inhibition on EPCR of HKC.Conclusion It was suggested that the expression of EPCR in HKC could be suppressed by TNF-α.Simvastatin may have a protective effect on the renal tubular epithelial cell injury by inhibiting the effect of TNF-α on EPCR.
出处
《苏州大学学报（医学版）》
CAS
2012年第1期54-57,共4页
Suzhou University Journal of Medical Science
基金
张家港市2010年科技发展计划项目(ZKS1008)
关键词
内皮细胞蛋白C受体
肿瘤坏死因子-Α
辛伐他汀
endothelial protein C receptor（EPCR）
tumor necrosis factor alpha（TNF-α）
Simvastatin
