高效液相色谱法测定柑橘皮中新橙皮苷

HPLC Determination of Neohesperidin in Citrus Peels

提出了高效液相色谱法测定柑橘皮中新橙皮苷含量的方法。样品用甲醇超声提取,定容至50mL。以SinoChrom ODS-BP色谱柱(250mm×4.6mm,10μm)为分离柱,以甲醇-水(36+64)溶液作为流动相,用紫外检测器在波长283nm处进行测定。新橙皮苷质量浓度在0.005 3～0.32g.L-1范围内与其峰面积呈线性关系,方法的检出限(3S/N)为0.6mg.L-1。方法的平均回收率为90.3%,相对标准偏差(n=6)为1.7%。

HPLC was applied to the determination of neohesperidin in citrus peels.The Sample was ultrasonically extracted with methanol,and made up to 50 mL.SinoChrom ODS-BP（250 mm×4.6 mm,10 μm） column was used as stationary phase,a mixture of methanol and water mixed in the ratio of 36 to 64（by volume） was used as mobile phase;UV-detection at 283 nm was used in the determination.Linear relationship between values of peak area and mass concentration of neohesperidin was obtained in the range of 0.005 3-0.32 g·L-1,with limit of detection（3S/N） of 0.6 mg·L-1.Values of average recovery and RSD（n=6） found were 90.3% and 1.7% respectively.