育龄妇女支原体培养假阳性及药敏干扰因素分析

Analysis on False-positive Result of Mycoplasma Culture and Interference Factors of Its Drug Sensitivity Test in Women of Childbearing Age

目的探讨育龄妇女支原体培养假阳性及药敏干扰因素的关联。方法支原体培养及药敏实验等所有操作按照《全国临床检验操作规程》第三版进行,支原体培养基澄清变红者,再做聚合酶联反应(PCR)鉴别假阳性,出现长菌者,需进行菌种鉴定及药敏实验。结果 684例育龄妇女中,支原体培养基澄清变红326例,由PCR诊断鉴别假阳性22例,支原体感染真阳性304例;菌种鉴定中,白色念珠菌202株,金黄色葡萄球菌2株,表皮葡萄球菌15株;菌株药敏实验差异明显。结论支原体培养48 h后培养基变红且澄清者,建议再做PCR支原体检测及细菌培养,排除支原体假阳性而误诊。

Objective To explore the correlation between the false-positive result of mycoplasma culture and the interference factors of its drug sensitivity test in women of reproductive age. Methods All operation steps of mycoplasma culture and drug sensitivity test were performed in accordance with the third edition of the National Clinical Laboratory Procedures.Polymerase chain reaction（PCR） was used to re-identify falsepositive results when the culture mediums of mycoplasma became ruddy and the supernatant was clear.Bacterial species identification and drug sensitivity test were performed when the bacteria were grown. Results Among the 684 surveyed women of reproductive age,mycoplasma mediums of 326 cases became ruddy and with clear supernatant layer after the culture,in which 22 cases were false-positive and 304 cases were mycoplasma infection identified by PCR.The results of bacterial species identification showed that there were 202 strains of Candida albicans,2 strains of Staphylococcus aureus,and 15 strains of Staphylococcus epidermidis.Antimicrobial susceptibility testing for the strains showed the differences were significant. Conclusions The results suggest that mycoplasma PCR test and bacteria culture are necessary when culture medium becomes ruddy and with clear supernatant fluid after 48 hours of mycoplasma culture so as to exclude the misdiagnosis caused by false-positives.