硫化氢在大鼠急性腹腔感染早期中的作用

The Role of Hydrogen Sulfide in Prophase of Acute Peritoneal Cavity Infection of Rat

目的探讨硫化氢(H2S)在大鼠急性腹腔感染早期中的抗炎作用。方法以硫氢化钠(NaHS)作为H2S供体,将72只SD大鼠随机分为空白对照组、盲肠结扎穿刺(CLP)+生理盐水(NS 1 ml/kg)组、CLP+NaHS组和CLP+炔丙基甘氨酸(PAG,一种抑制体内产生H2S的酶的试剂)组,每组各18只。模型制作成功后经大鼠尾静脉注射给药,每组分别于给药后2、6和12 h各随机选取6只大鼠,检测血清中TNF-α和H2S的含量以及造模6 h回肠组织中髓过氧化物酶(MPO)的含量,并观察回肠组织的病理学改变。结果 H2S能明显减轻CLP模型组中的炎症反应,降低炎症反应时血清TNF-α的含量,减轻中性粒细胞在小肠组织中的浸润。结论 H2S在大鼠急性腹腔感染早期具有抗炎作用。

Objective To study the role of hydrogen sulfide（H2S）in prophase of acute peritoneal cavity infection.Methods NaHS was taken as a donor of H2S.Seventy-two Sprague-Dawley rats were divided into 4 groups randomly：control group,cecal ligation and puncture（CLP）and treated with natural saline group,CLP and treated with NAHS group,and CLP and treated with DL-propargylglycine（PAG,an inhibitor of H2S formation）group.Selected 6 rats at 2 h,6 h,and 12 h after treatment in each group.The contents of TNF-αand H2S in serum and the content of MPO in intestinal tissue were measured,respectively.The histopathological change of ileum tissues were observed at 6 h after treatment in each group.Results The H2S could alleviate CLP-induced inflammation obviously,decrease the content of TNF-α in serum when inflammation,and attenuate the infiltration of neutrophilic granulocyte in small intestine.Conclusion The H2S has anti-inflammation effect in prophase of acute peritoneal cavity infection.