依达拉奉预给药对油酸致急性肺损伤大鼠的作用及机制

Effects of edaravone pretreatment on acute lung injury in rat model of injected oleic acid

目的探讨依达拉奉预先给药后,对油酸致急性肺损伤(acute lung injure,ALI)模型大鼠的作用及机制。方法雄性SD大鼠30只,随机分为3组(n=10):对照组(A组)腹腔注射10mL/kg的0.9%氯化钠注射液后,鼠尾静脉注射0.9%氯化钠注射液0.1mL/kg;ALI组(B组)腹腔注射10mL/kg的0.9%氯化钠注射液后,静脉注射油酸0.1mL/kg制备ALI模型;依达拉奉组(C组)腹腔注射10mL/kg的依达拉奉后同B组方法建立ALI模型。6h后处死大鼠,抽取腹主动脉血液行血气分析测定血氧分压(PO2),用ELISA法测定血液中肿瘤坏死因子-α(TNF-α)、丙二醛(MDA)、一氧化氮(NO)的含量及肺组织中超氧化物歧化酶(SOD)的含量,分离大鼠右肺中叶,测定肺组织湿/干重比(W/D)。结果与A组相比,B、C组大鼠腹主动脉血PO2、肺组织SOD含量均低于A组,差异有统计学意义(P<0.05)。C组PO2、SOD低于A组,但是其降低的幅度小于B组,与B组相比,C组PO2、SOD高,且差异有统计学意义(P<0.05)。A组W/D、TNF-α、NO、MDA的含量均低于B、C组;B组W/D、TNF-α、NO、MDA的含量均增加明显,与A组比较,差异有统计学意义(P<0.05);C组W/D、TNF-α、NO、MDA的含量增加幅度小,与B组比较,差异有统计学意义(P<0.05)。结论预先注射依达拉奉不能减少肺损伤的发生率,但可减轻油酸诱导的大鼠急性肺损伤程度,其机制可能与清除氧自由基和抑制炎性因子释放有关。

Objective To investigate the effect of edaravone pretreatment on acute lung injury in rat model of injected oleic acid. Methods Thirty SD rats were randomly divided into 3 groups （n = 10）： group A （sham operated group）, group B （ALI group）, group C （edaravone group）. Group A received intraperitoneal injection of normal saline 10 mL/kg, than prepared model by intravenous injection normal saline 0.1 mL/kg. Group B received intraperitoneal injection of normal saline l0 mL/kg, than prepared model by intravenous injection OA 0.1 mL/kg. Group C received intraperitoneal injection of edaravone 10 mL/kg, than prepared model by intravenous injection OA 0.1 mL/kg. PO2 of blood in the aorta abdominalis were mea sured. The expression of TNF-c~, NO, MDA, SOD was examined by ELISA. Wet/dry weight of lung tissue was calculated. Results The levels of POz and SOD in group A were more than that in group B and C. The increase in the levels of POE and SOD in groups C was more than that in group B （P 〈 0.05）. The levels of W/D, TNF-α, NO, MDA in group A were less than that in group B and C. There were significant differences in the levels of W/D, TNF-α, NO, MDA between groups B and A （P 〈 0.05）. And compared with group B, the increase in the levels of W/D, TNF-α, NO, MDA in groups C was less than that in group B （P 〈 0.05）. Conclusion Pretreatment with edaravone can attenuate ALI by scavenging free radical and inhibiting proinflammatory cytokines.