NFκB1基因启动序列-94ins/delATTG多态性与社区获得性肺炎易感性及严重程度相关性研究

Association of NFκB1-94ins/delATTG promoter polymorphism with the predisposition to or severity of community acquired pneumonia

目的探讨NFκB1基因启动序列-94ins/del ATTG基因多态性与中国广东省汉族人群社区获得性肺炎(CAP)易感性及严重程度的相关性。方法采用聚合酶链反应-高分辨率熔解曲线法(PCR-HRM)检测66例CAP患者和66例健康体检者的NFκB1基因启动序列-94ins/del ATTG基因型的分布,分析该基因多态性与CAP易感性及严重性的相关性。结果 NFκB1基因启动序列-94ins/del ATTG基因型和等位基因分布频率在CAP组和健康对照组比较差异无统计学意义(P>0.05);在重症肺炎组与非重症肺炎组比较差异无统计学意义(P>0.05);NFκB1-94ins/del ATTG的不同基因型与CAP患者的性别、年龄、住院时间、白细胞数、中性粒细胞数之间的相关性均无统计学意义(P>0.05)。结论 NFκB1基因启动序列-94ins/del ATTG多态性与CAP易感性及严重程度无相关性。

Objective To determine the association of NFnBI - 94ins/delATTG promoter polymorphism with the predisposition to or severity of community acquired pneumonia （CAP） in Chinese Han population in Guangdong. Methods Polymerase chain reaction-high resolution melting curve （PCR-HRM） was used to analyze the polymorphism of NFκB1 - 94ins/delATTG pro-moter in 66 CAP patients and 66 healthy controls. Results No significant difference was found between CAP group and control group （P〉0.05）, or between severe CAP （SCAP） group and non-SCAP group （P〉0.05） in the genotype distribution and allele frequency. Any genotype of NFκBI -94ins/delATTG promoter was not associated significantly with patient gender, age, or days of hospital stay, WBC or neutrophil count in CAP patients. Conclusions Our findings indicate that NFκB1 - 94ins/de1ATTG promoter polymorphism is not associated with the predisposition to, or severity of community acquired pneumonia.