斜纹夜蛾前胸腺的生长发育研究

Research on the Development of Prothoracic Glands of Spodoptera litura

通过解剖观察4龄至蛹期斜纹夜蛾前胸腺,对其形态特点和生长发育规律进行了研究,主要结果如下:发现斜纹夜蛾前胸腺由53个左右的圆形或椭圆型细胞成串组成,Y型,成对,外围包裹一层透明鞘膜,位于胸部第一气门的气管丛内;在斜纹夜蛾4龄到蛹期的发育过程中,前胸腺不发生有丝分裂,细胞数量基本恒定;前胸腺细胞直径在幼虫发育期间不断增大,斜纹夜蛾幼虫在4龄第1天的前胸腺直径为(28.80±0.47)μm,而到了6龄末期,则增加到(92.22±2.43)μm;幼虫期的斜纹夜蛾前胸腺细胞直径和虫体体质量呈显著相关,r=0.826,P=0.000,拟合曲线方程为Y=82.1X0.263;此外,幼虫期的斜纹夜蛾前胸腺细胞直径和虫体体长也呈现出显著相关,r=0.886,P=0.000,拟合曲线方程:Y=e(4.781-1.863/Z)。

The morphology and regular pattern of prothoracic glands of Spodoptera litura were by dissecting these glands from the 4th instar larvae to pupation. The result showed that the glands of S. litura were in pair with Y-type, consisted of 53 round or oval cells, surrounded researched prothoracic with trans-parent vaginal tunica, and located in the tracheal plexus of the first spiracle. The cells of prothoracic glands kept steady because of no mitosis from the 4th instar larvae to pupation. The diameter of prothorac- ic gland cells was increased gradually during larval developments, the diameter of prothoracic gland ceils from the larva at the first day of the 4th instar was （28.80 ±0. 47） μm, while the diameter of prothoraeic gland cells from the larva at the last day of 6 instar was （92.22 ±2.43 ）μm. There were significant rele- vance between the diameter of prothoracic gland cells and the weight of larva during the larval develop- ment, r = 0. 826, P = 0. 000, Y = 82. 1X^0. 263. There were also significant relevance between the diameter of prothoracic gland cells and the body length of larva during the larval development, r = 0. 886, P = 0. 000, Y=e=e^（4.781-1.863/Z）.