促红细胞生成素对脑出血大鼠血肿周围胶质原纤维酸性蛋白表达的影响

THE EFFECTS OF ERYTHROPOIETIN ON GFAP EXPRESSIONS OF ASTROCYTES IN THE RATS WITH CEREBRAL HEMORRHAGE

目的探讨促红细胞生成素(EPO)对大鼠脑出血后脑水肿和胶质原纤维酸性蛋白(GFAP)表达的影响。方法将60只SD大鼠随机分为EPO组、对照组和假手术组。EPO组及对照组用注射胶原酶及肝素的方法制作SD大鼠苍白球脑出血模型,EPO组向腹腔内注射EPO,治疗剂量为3000U/(kg.d),对照组不注射药物。采用干湿比重法测定各组脑含水量,应用免疫组织化学方法检测各组脑组织GFAP的表达与变化。结果对照组和EPO组在脑出血后出现脑含水量的增高,至第3天达高峰,随后逐渐减少,对照组含水量高于EPO组(P<0.01或0.05);对照组和EPO组在出血后第1天即可见出血灶周围GFAP的高表达,至第3天达高峰,随后逐渐下降,至第7天时仍高于假手术组,对照组GFAP的表达高于EPO组(P<0.01);血肿周围GFAP的表达水平与水肿体积呈正相关(r=0.861,P<0.01)。结论 EPO可能通过抑制胶质细胞足突上GFAP的表达减轻脑出血后的脑水肿。

Objective To observe the effects of erythropoietin（EPO） on cerebral edema and the expressions of glial fibrillary acidic protein（GFAP）of astrocytes in the rats with cerebral hemorrhage.Methods The models of intracerebral hemorrhage（ICH）were established by infusing collagenase and heparin into right globus pallidus of SD rats.SD rats were randomized into the EPO group,the control group and the sham operation（SO）group.The wet-dry weight proportion evaluation method was used to calculate the cerebral water content.Immunohistochemistry was used to detect the GFAP expressions.Results The cerebral water contents increased and reached peak in the third day and then decreased gradually in both the contrast group and the EPO group,with the water content being higher in the contrast group than in the EPO group（P0.01或0.05）.In the contrast group and the EPO group,on the first day of cerebral hemorrhage,the GFAP expressions were high and reached the peak value on the third day and then decreased gradually,but still higher than in the SO group on the seventh day with the GFAP expressions higher in the contrast group than in the EPO group（P0.01或0.05）.The GFAP expressions were in positive correlation to the volume of edema（r=0.861,P0.01）.Conclusion EPO may restrain the expressions of GFAP to relieve cerebral edema in rats with cerebral hemorrhage.