1,25二羟维生素D_3对哮喘大鼠气道重塑及纤溶酶原激活物抑制剂-1的影响

Effects of 1,25(OH)_2D_3 on PAI-1 in Asthma Rats of Airway Remodeling

目的观察1,25二羟维生素D3(VD)对哮喘大鼠气道重塑及其肺组织中纤溶酶原激活物抑制剂-1(PAI-1)表达和血浆中PAI-1含量的影响。方法 30只健康雄性Wistar大鼠随机分为对照组、哮喘组、VD干预组,每组各10只。卵蛋白致敏和激发复制慢性哮喘模型。VD干预组每次激发前给予VD干预。用免疫组化检测肺组织PAI-1的表达,酶联免疫法测血浆中PAI-1含量,采用图像分析进行图像分析。结果 (1)哮喘组支气管管壁厚度较对照组和VD干预组显著增加(P<0.01)。(2)哮喘组PAI-1在大鼠肺组织的表达程度较对照组和VD干预组显著增加(P<0.01)。(3)哮喘大鼠血浆中PAI-1含量较对照组和VD干预组明显增加(P<0.01)。(4)直线相关性分析显示,哮喘组支气管管壁厚度与大鼠肺组织中PAI-1表达水平呈正相关(r=0.822,P<0.01);哮喘组支气管管壁厚度与大鼠血浆中PAI-1含量呈正相关(r=0.942,P<0.01)。结论 1,25二羟维生素D3干预可明显减轻慢性哮喘气道重塑的病理改变,并可通过部分抑制PAI-1的表达来延缓气道重塑。

Objective To observe the effects of 1,25（OH）2D3 on the airway remodeling and the expression of PAI-1 in asthma rats.Methods Eighteen wistar rats were randomly divided into three group：the control group,the asthma model group,and the 1,25（OH）2D3 group.The asthma model was established by sensitization and challenged with ovalbumin,and the rats in the 1,25（OH）2D3 group injected 1,25（OH）2D3 before the challenge.The following parameters were measured： the protein expression of PAI-1 by immunohistochemistry and the level of PAI-1 by ELISA.the bronchial wall thickness by computer-assisted image analysis system.Results（1）the bronchial wall thickness in the asthma group was higher than in the other groups（P〈0.01）.（2） The expression of PAI-1 in the asthma group higher than that in other groups（P〈0.01）.（3）The lever of PAI-1 in plasma of asthma group was higher than that in other groups（P〈0.01）.（4）There was a positive correlation between the activity of PAI-1 in rat lungs and the bronchial wall thickness（r=0.822 P〈0.01）,the same between the level of PAI-1 in plasma and the bronchial wall thickness（r=0.942 P〈0.01） Conclusion 1,25（OH）2D3 could decrease the expression of PAI-1 in lung tissues of asthmatic rats and the level of PAI-1 in plasma and inhibit airway remodeling.