人大肠癌鸡胚尿囊膜移植模型的建立及血管生成特征的观察

Establishment of a Human Colorectal Cancer Model on the Chick Embryo Chorioallantoic Membrane

目的研究人大肠癌腺癌细胞株HT-29鸡胚尿囊膜移植模型建立的方法,观察和分析其血管生成的特征。方法将不同浓度的人大肠癌腺癌细胞株HT-29接种于鸡胚尿囊膜(chick embryo chorioallantoic membrane,CAM),观察影响大肠癌鸡胚移植模型瘤体成活的因素、瘤体生长特征和血管生成情况。结果建立了人大肠癌CAM移植模型。移植模型瘤体易于生长,具有较强的血管生成作用。结论该模型易于复制,能动态观察大肠癌的血管生成过程,可用于大肠癌的生物学行为、药物筛选等领域的研究。

Objective To establish a human colorectal cancer model on the chick embryo chorioallantoic membrane（CAM） and study its characteristics of angiogenesis.Methods Human colorectal cancer cell line HT-29 at different concentrations were inoculated on the CAMs.To observe the factors affecting the survival of the transplanted human colorectal cancer,growth characteristics of the transplanting tumor and the characteristics of angiogenesis.Results The transplantation tumor model of human colorectal cancer in CAM was successfully established.It was found that transplantation tumor was easy to grow and it showed strong angiogenesis effects.Conclusions It is feasible to establish a transplantation tumor model of human colorectal cancer on CAM.The model can be easily duplicated,which provides a useful animal model for studying the angiogenesis and biological properties of colorectal cancer and can be used in the research of the drug selection for human colorectal cancer.