不同佐剂H3N2亚型猪流感灭活疫苗的免疫效果比较

Comparison of Immune Effect of H3N2 Swine Influenza Virus Inactivated Vaccine With Two Different Adjuvants

选用福建分离猪流感A/Swine/Fujian/F1/03(H3N2)毒株为制苗种毒,制成2种不同佐剂灭活疫苗。挑选H3N2亚型猪流感抗体阴性的母猪和断奶仔猪用于检测比较2种灭活疫苗的免疫效果。结果表明:IMS1315佐剂灭活疫苗首免母猪和断奶仔猪7d后均可检测到HI抗体,二免28d后抗体水平最高,其中母猪的抗体均值为11.0Log2,断奶仔猪的抗体均值为10.2Log2,二免150d后仍维持在较高抗体水平,均值在6.5Log2以上;医用白油佐剂灭活疫苗首免母猪和断奶仔猪14d后均可检测到HI抗体,二免28d后抗体水平达最高,其中母猪的抗体均值为10.2Log2,断奶仔猪的抗体均值为10.0Log2,二免150d后抗体水平均值在6.0Log2以上。表明2种不同佐剂灭活疫苗不论免疫母猪还是仔猪,均具有较长的抗体消长期,但IMS1315佐剂灭活疫苗产生抗体略好于医用白油佐剂灭活疫苗。

H3N2 swine influenza virus inactivated vaccines were prepared with IMS1315 adjuvant and oil emulsion adjuvant.Sows and piglets,which were antibody negative of H3N2 swine influenza virus,were selected and vaccinated two times at intervals of 21days with both inactivated vaccines.Antibodies were determined 12 times at the immune period of 171days with HI.The results showed that both in sows and piglets,the HI positive antibodies were detected at the 7th day after vaccination with IMS1315 adjuvant vaccine and were detected at the 14th day after vaccination with oil emulsion vaccine.The antibody peak levels were detected at the 28th day after second vaccination of both inactivated vaccines.They were 11.0 Log2,10.2 Log2 in sows and 10.2 Log2,10.0 Log2 in piglets,respectively.The antibody levels could be maintained in 6.5Log2 and 6.0Log2 at the 150th day after the second vaccination,respectively.It indicated that the effect of IMS1315 adjuvant inactivated vaccine was better than that of oil emulsion inactivated vaccine.