水山药“九斤黄”组织培养及微型块茎诱导

Tissue Culture and Micro-tuber Induction of Dioscorea opposita,Jiu Jin Huang

利用山药珠芽进行种茎繁殖,对山药品种具有更新复壮作用;为降低山药种植成本,促进山药品种更新复壮,探索适宜水山药品种"九斤黄"微型块茎诱导的培养条件。通过不同浓度NAA/6-BA激素组合对茎尖诱导的作用,以及不同浓度NAA/KT激素组合、不同活性炭浓度、不同蔗糖浓度处理,筛选适宜微型块茎诱导的配方条件。结果表明:MS培养基中添加0.1mg.L-1 NAA+2.0mg.L-16-BA为水山药"九斤黄"茎尖诱导最佳浓度,植株再生率达到了91.7%;"九斤黄"微型块茎诱导最适宜培养基配方为:MS基本培养基+0.1mg.L-1 NAA+1.0mg.L-1 KT+2.0g.L-1 AC+50.0g.L-1蔗糖+8.0g.L-1琼脂粉。再生微型块茎的种植仍然保持原品种种性,但外观商品性获得提高。

Using yam bulbils for tuber propagation not only benefits the rejuvenation of Dioscorea opposita but also reduces the planting cost.Thus,conditions of the micro-tuber induction for the yams were studied.The shoot tips were cultured in MS medium with additions of varying concentrations of NAA/6-BA.Different treatments using NAA/KT,active carbon and sucrose were applied for the experimentation.The results indicated the optimal medium composition for the shoot tip induction to be： MS ＋ 0.1 mg·L-1 NAA＋ 2.0 mg·L-1 6-BA＋ 0.2 g·L-1 AC＋ 30.0 g·L-1 sucrose＋ 8.0 g·L-1 agar.The resultant plant regeneration rate was 91.7%.The optimal medium formula for the micro-tuber induction was： MS ＋0.1 mg·L-1 NAA＋ 1.0 mg·L-1 KT＋ 2.0 g·L-1 AC＋ 50.0 g·L-1 sucrose＋ 8.0 g·L-1agar.The regenerated plants from the micro-tubers not only maintained the characteristics of the parent species but also improved its commercial appealing.