ISSR、RAPD和SRAP分子标记技术在姬松茸菌株鉴定上的应用比较

DNA Fingerprinting and Genetic Diversity of Cultivated Strains of Agaricus blazei Murill

利用ISSR、RAPD和SRAP3种分子标记法对16株姬松茸菌株进行比较分析。结果表明其中8条RAPD、4条ISSR和2对SRAP引物适合姬松茸菌株鉴定分析,3种标记方法均将16个菌株分为3大类群,A0011+1和A0013为1类,A0009单独为1类,其余菌株为1类。3种分子标记法对姬松茸菌株鉴别的结果存在着一定差别。SRAP反应的遗传信息较丰富,比ISSR、RAPD检测到更大的遗传差异;RAPD引物扩增到的多态性条带数较多。

Sixteen different strains of Agaricus blazei Murill were analyzed using ISSR,RAPD and SRAP.PCR amplification using 8 RAPD primers,4 ISSR primers and 2 SRAP primer pairs separated the strains into three groups.One of them consisted of A0011＋1 and A0013,another A0009,and the third group the remainders.The result showed that SRAP provided rich genetic information,and the RAPD primers had the largest number of polymorphic bands among all markers.